The mapping population was genotyped using the Illumina Infinium 15 k Wheat platform. 700 μl of chloroform/isoamyl alcohol (24:1 vol:vol) was added.QTL analysis allowed us to identify loci covering 78 SNP markers in the pericentromeric region of 5B chromosome significantly ( (CS-5Bdic) and F1 seeds of CS x CS-5Bdic were kindly provided by Prof. After centrifugation (15 min, 12000 rpm, Eppendorf 5415 R), the supernatant was collected in new tube and 1.4 ml of 96 % cold (−20 °C) ethyl alcohol was added and mixed gently.The reported nucleotide sequences of alleles from cv.Chinese Spring and substitution line CS-5Bdic were deposited in the EMBL, Gen Bank, and DDBJ nucleotide sequence databases under accession numbers [Gen Bank: KT750252] and [Gen Bank: KT750253], respectively. The plants were grown in a greenhouse (20 °C-25 °C) under 12 h of light per day without vernalization.Several of the releases listed below can be found at Unearthed Music or online music retailers such as Amazon and i Tunes.
The album is full of thick analog arpeggios, punchy bass lines, earthy beats, and ballistic leads.
Artist: Keston and Westdal Label: Unearthed Music Release Date: Tuesday, March 25, 2008 This is the third album produced by Keston and Westdal.
“Every now and then, an act releases an album changing the way we listen to music. Slackline calls it, “…the kind of stuff that will have a high value for travelers on future long voyages to Mars.” Artist: Keston and Westdal Label: Unearthed Music Release Date: Tuesday, March 25, 2008 Keston and Westdal’s second full-length title on Unearthed Music features collaborations with Steven Ekoka, Estaire Godinez, Desdamona.
Using the Illumina Infinium 15 k Wheat platform, 379 5B-specific polymorphic markers were detected and a genetic map with 82 skeletal markers was constructed.
Phenotype (heading date) – genotype association analysis revealed seventy eight markers in pericentromeric region of 5B chromosome significantly associated with heading date variation.